Tamaraty | PhD life 🧫🌸💕
Region: US
Monday 16 June 2025 21:37:53 GMT
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kineticpicachu :
i've done hundreds, the trick is to actively swear along the whole process. The blot senses fear, so teach it how to feel it too
2025-06-18 23:04:20
879
neah.elizabeth :
I swear it SMELLS fear, I refuse to do them lol
2025-06-16 23:03:10
878
A :
I moved from western blots to a Jess Simple western. The best thing my Pi ever bought
2025-07-27 06:14:27
1
faith aurelia :
as a literal graduated bio major who has had bio profs teaching this, you deserve one million dollars thank u
2025-06-16 22:16:42
478
elizabeth :
did a western blot once. took 14 hours
2025-06-17 12:57:09
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kaiTpain :
Why not do an ELISA
2025-07-24 05:06:11
0
BigPP :
yall denaturing samples in a waterbath?
2025-06-17 05:43:39
0
Maria Vanessa :
but you're not wearing your western gear ! 🤠
2025-06-18 11:52:41
47
Heidi Satterfield18 :
Literally cannot fathom creating something like this.
2025-07-25 01:13:54
0
Maggie :
Thank you for explaining this so well and showing the process!!!! This is so helpful for understanding how the whole blotting process works, I'm going to take the mcat at the end of the year😁
2025-07-12 11:37:05
1
Molly :
Do you have examples of a finished western blot?
2025-06-16 22:12:44
26
user5583058612711 :
How is the antigen detected by the antibody if the sample protein is denatured by SDS?
2025-06-16 22:49:11
1
Ad :
Whats the difference between western blot and gel electrophoresis?
2025-06-17 08:11:50
7
Neili🥑🪴 :
I HATE THE betamercaptethanol part sooooo much
2025-06-21 20:12:07
0
tangina_nyo_lahat0 :
What job does this
2025-06-19 16:07:34
0
-_- :
You explained this so well thank you
2025-06-16 22:55:51
49
victorlagazan :
How do people find this hard😂. Wait untill you see labday of synthetic organic chemistry
2025-06-23 16:55:18
0
blobby :
Love love love doing Westerns! Seeing snatched, crispy, single bands makes me feel like a master of black magic.
2025-06-20 01:31:10
0
Khristina C. :
When I first did one learning you have to start early or you weren’t leaving the lab any time soon 😭😭
2025-06-20 05:32:33
1
InspiredUG :
Not me watching this with my samples incubating in primary antibody overnight and I am hoping it doesn’t disappoint me by morning.
2025-06-26 04:17:04
14
Mikhaela :
I’d rather do WB than PCR 😬
2025-06-17 08:31:19
3
workingmomlife :
I love western blots. I’m in biotech and I run around 120 gels a month. we utilize transblot turbos for semi-dry transfer.
2025-06-23 01:57:21
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Green :
i watched the whole thing even tho i have done countless western blots
2025-06-18 15:55:22
1
Sam :
step 1. say the swear prayer step 2. process the western step 3. repeat the swear prayer 😂😂
2025-06-21 00:18:14
13
se_149 :
I did so many of these. Spent so many hours in the dark room having panic attacks that it wouldn’t work 🤣 I once had such a strong signal it glowed in the dark lol
2025-06-21 10:59:41
0
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